What is PHYTEX 500
Phytase is an enzyme found in nature mainly in plants . It is formed in the stage of sprouting to supply phosphorous during plant growth. Phytase is also found in the small intestine mucous of many animals, and in case of micro-organisms, it is found in Aspergillus sp. and yeast. Its is obtained by a NON-GMO natural process from Aspergillus Nнger.

Mode of action
Inositol Hexaphosphate , (IH), present in grains and beans, contain one to three percent of (IH) , of which, 60% to 80% is phosphoric acid. Wheat:0.6 to 1.4 % (w/w); Bran:2.6 to 5.4 % (w/w) ; Soy Flakes:1.5 to 1.8% (w/w) Prot. Concr: 2.0 to 3.0% (w/w) Phytase breaks down the (IH) molecule releasing its phosphoric acid content making it bioavailable to the animal, thus reducing the need for imorganic phosphate.

PHYTEX 500 Savings in formulation, economic benefits and units of activity
PHYTEX 500 when added to feed at the recommended levels, allows for savings of 1.5% protein and 0.1 % phosphorous in formulation. This represents a savings of at least 600% its cost of inlusion per Mt of feed. One unit of activity of phytase in PHYTEX 500 is the amount of enzyme required to liberate 1 micromole of inorganic phosphorous per minute under the conditions of its assay method. . One AGR U* (unit of activity) is the amount of enzyme required to produce,together, an increase of 0.1% (w/w) of liberated phosphorous and an increase of 1.5 % ( w/w) soluble protein in a gram of standard formulated feed ration, at pH 5 , at 37єC. PHYTEX contains 5000 AGR U* per gram.

Other benefits:
Inositol Hexaphosphate (IH) , binds minerals, such as iron, calcium, magnesium in feed, diminishing their bioavailability. It also forms an undissolved complex with protein which prevents activity of digestive enzymes, decreasing digestability of proteins and their functions, such as emulsifying.Phosphoric acid in (IH) can not be used by single stomach animals. Therefore, the need to add inororganic phosphate in feeds as an additive. However, (IH) is secreted in feces, creating environmental problems in large swine operations.

In the Netherlands, the use of poultry manure as a fertilizer is regulated per regards to the amount of phosphate (P2O5) per acre.

Rate of application
100 grams per MT of feed. It can be pre-mixed or dispensed directly into the mixer without pre-mixing. This inclusion rate provide 500,000 AGR U* units per Mt of feed.

Soluble in water:

Finisher 100 grms (Phytex 500) dissolved in 1000 Litres of water for 500 pigs

Grower 100 grms (Phytex 500) dissolved in 1000 Litres of water for 1000 pigs

Poultry 100grms(Phytex 500) dissolved in 1000 Litres of water for 10,000 Chickens.


Charcteristics
Activity Vrs. Temperature: 100% Act.@ 60 єC. ( Range 48єC to 68єC ) . Activity Vrs. pH : 100% Act. @ pH 6. (Range from 5.5 to 7). However, PHYTEX 500 can be pelletized at maximum: 80 є C for 30 seconds. max with an aproxнmate 5 % to 10% l enzyme loss. It is 100 % soluble in water. For maximum effect, add after pelleting.

Packaging
PHYTEX 500 is packed for bulk shipments in 20 kg boxes lined with special poly- plastic bag.It can also be packed in smaller polybags as required by customer-




AMMINOGRAM INTERPRETATION
- The hydrolytic action of PHYTEX 500 on innositol hexaphosphate increases the bio-availability of phosphorous, as well as other nutrients in feeds. The presence of inositol hexaphosphate binds minerals and forms an undissolved complex with proteins, diminishing the bioavailability of nutrients in feeds. The breaking up of inositol hexaphosphate by PHYTEX 500, not only releases phosphorous , but a number of other nutrients , as expressed by the accompanying aminogram.

The values that appear in the amminogram are in terms of percentages and only take into account the hydrolytic action of PHYTEX 500 . The percentages are directly related to the PHYTEX 500 rate of inclusion in the feed ( grams / Mt of feed).

In order to determine the % additional value of each nutrient made available to the animal due to each enzyme's hydrolytic action , the following formula should be used:

Thus the nutrional value for each nutrient in % per Mt of feed equals:

Making this calculation for each nutrient in the amminogram , will determine the total savings in formulation by PHYTEX 500 .






METHOD OF ASSAY TO DETERMINE AGRI*U ( UNITS)

DEFINITIONS:
AGRI*U definition:

• An AGRI*U (Unit) is the amount of PHYTEX 500 required to produce, together, an additional 1.5% of soluble protein and an additional 0.1% free phosphorous in feed rations.
• One gram of PHYTEX contains 5,000 units of AGRI*U

MODE OF ACTION
The hydrolytic action of PHYTEX 500 on inositol hexaphosphate (IH) increases the bioavailabilty of phophorous , as well as proteins in feed rations. Inositol hexaphosphate (IH) binds minerals and undissolved complexes (that bind proteins), diminishing the bioavailability of these proteins.in the animal gut. The breaking up of (IH) by PHYTEX 500 releases free phosphorous and unbinds these protein, making them more bioavailable to the animal. This action allows for formulating rations with less phosphorous and less protein, producing a savings in said formulation.


METHOD OF ASSAY
The object of the method of assay is to determine the increase of free phosphorous and soluble protein in the ration in the ration.

1) Take one 2 kg of poultry grower feed and mix in two separate jars , each, with a 1 liter solution ( Ph 5) simulating the gastric juice composition.
2) In one jar, apply 1 (one) gram of PHYTEX 500.
3) The other jar (control) , stays blank.
4) Incubate both jars for 24 hours, at gut temperature while stirring mildly and continously .
5) After 24 hours analyze for free phosphorous by means of a spectrometer and for soluble protein.